Cross-talk between CDK4/6 and SMYD2 regulates gene ... - Science
WEBOct 30, 2020 · In this study, we identify a direct connection between CDK4/6 and the epigenome, in that CDK4/6 directly interacts with and phosphorylates SMYD2 (Fig. 1) to regulate SMYD2-mediated histone H3K4 or H3K36 methylation (Fig. 2), which either promotes or represses gene transcription, respectively.
CDK5–cyclin B1 regulates mitotic fidelity - Nature
WEBSep 4, 2024 · CDK1 has been known to be the sole cyclin-dependent kinase (CDK) partner of cyclin B1 to drive mitotic progression 1. Here we demonstrate that CDK5 is active during mitosis and is necessary for...
Development of an orally bioavailable CDK12/13 degrader ... - Cell …
WEB2 days ago · CDK12 and its paralog CDK13 belong to the transcriptional cyclin-dependent kinase (CDK) ... Tubulin is the loading control probed on all immunoblots. (D) Real-time growth curves of VCaP cells upon treatment with siCDK12/13 and/or uprosertib. Data are presented as mean ± standard deviation from three independent experiments.
Microtubule Regulation in Mitosis: Tubulin Phosphorylation by …
WEBDec 21, 2005 · The cyclin-dependent kinase Cdk1 (or Cdc2), associated with its cognate partner cyclin B, is a key enzyme for entry in mitosis (Nigg, 2001) and is essential for spindle morphogenesis. Cdk1 up-regulates microtubule dynamics when added in cell-free Xenopus extracts (Verde et al., 1990, 1992).
The Hydrophobic Patch Directs Cyclin B to Centrosomes to …
WEBMar 3, 2020 · Our experiments suggest that the hydrophobic patch of mitotic cyclins contributes to CDK substrate selection by directing the localization of Cdc13-CDK to centrosomes and that this localization of CDK contributes to the CDK substrate phosphorylation necessary to ensure proper entry into mitosis.
B1‐type cyclins control microtubule organization during cell division ...
WEBDec 9, 2021 · Introduction. A highly elaborated control system guides cells through mitosis during which chromosomes are separated and distributed to the newly forming daughter cells. Cyclin‐dependent kinase (CDK)‐cyclin complexes stand in the center of this control system (Morgan, 1997; Lindqvist et al, 2009).
Live-cell imaging defines a threshold in CDK activity at the G2/M ...
WEBFeb 26, 2024 · FRET imaging with Eevee-spCDK demonstrated that in vegetatively growing S. pombe cells, CDK activity—or the balance between CDK activity per se and related phosphatases—increases at the time of the S phase, then gradually rises during G2 to reach a maximum level in the M phase.
Molecular hybrids: A five-year survey on structures of multiple ...
WEBDec 5, 2021 · Different kinase inhibitor agents which target EGFR, VEGFR, c-Met, CDK, PDK and other targets were merged into hybrids with conventional chemotherapeutics such as tubulin polymerization and topoisomerase inhibitors.
Identification of Cdk1–LATS–Pin1 as a Novel Signaling Axis in Anti ...
WEBMay 31, 2018 · Treatment with anti-tubulin drugs activated cyclin-dependent kinase 1 (CDK1), which phosphorylates LATS2 at five S/T-P motifs that functionally interact with the WW domain of Pin1 and inhibit its antiapoptotic function.
A novel landscape of nuclear human CDK2 substrates revealed
WEBApr 17, 2020 · In addition to known CDK substrates, we found a novel landscape of candidate CDK2 substrates, most of which function within key nuclear processes, including cell cycle control, histone modification and chromatin organization, DNA replication and repair, transcription, and RNA metabolism (Fig. 3A).