New One-Step Protocol Saves Time and Delivers Greater Consistency Traditionally, protein purification from E. coli includes four phases: harvest, bacterial cell lysis, lysate clarification, and ...

This proteomic protocol purifies and identifies palmitoylated proteins (i.e., S-acylated proteins) from complex protein extracts. The method relies on an acyl-biotinyl exchange chemistry in which ...

An effective protein extraction protocol must also be adaptable to the great variation in the sets of secondary metabolites and potentially contaminating compounds that occurs between tissues (e.g ...

The ability to obtain pure proteins is essential for developing drugs, creating vaccines and understanding biological processes. In this article, we discuss what a typical protein purification ...

Researchers from several institutions in Brazil developed an enhanced protocol for extracting and analyzing proteins from human skin, combining chemical and mechanical lysis, SP3 sample preparation, ...

Norgen's RNA/Protein Purification Plus Kit isolated both large RNA (represented by 28S and 18S rRNA) as well as small RNA with high integrity and without having to perform any additional protocol.

Scientists can choose from many protein purification protocols, but some methods fail to produce sufficient sample purity. By selecting the right chromatography method, researchers can optimize the ...

Coaffinity purification of two proteins from a complex mixture is one of the standard methods for the detection of protein-protein interactions. To circumvent the need for specific antibodies in ...

The new extraction protocol developed by Payne and Davis is an important contribution to USAMRIID’s ongoing effort to build serologic testing capacity in partner laboratories throughout AFRICOM ...

Studies of gene expression in plants and other organisms rely on the extraction of high-quality RNA. Although numerous protocols for RNA extraction have been developed, most of these are plant ...

Overview of Cell Lysis and Protein Extraction Summary Cell lysis refers to the process of breaking down the cell membrane to release cellular contents, including proteins, nucleic acids, and other ...